CRISPR-based detection is fast, we added accuracy.

We measure CRISPR’s activity on pre-edited DNA to quantify all types of genetic modifications, from large insertions or deletions to single nucleotide edits. The QUiCKR technology analyzes CRISPR’s kinetic rates to quantify edits with unprecedented speed and accuracy.

01

gRNA Probe Design

On-Target Modification

Custom gRNA Probe Optimization

02

CRISPR Kinetics Reaction

On-Target Site Detection

Fluorescent Reporter Cleavage

03

Kinetics Readout and Quantification

Fluorescence Progress Curves

Kinetics Analysis & Sequence Quantification

Patents and Publications

Enzyme kinetics analyses of CRISPR endonucleases 2023-04-12, Juan G. Santiago, Alexandre S. AVARO, Ashwin Ramachandran, Diego A. HUYKE, Charles BLANLUET

Blanluet, C., Huyke, D. A., Ramachandran, A., Avaro, A. S., & Santiago, J. G. (2022). Detection and discrimination of single nucleotide polymorphisms by quantification of CRISPR-Cas catalytic efficiency. Analytical Chemistry, 94(43), 15117-15123.

Blanluet, C., Kuo, C. J., Bhattacharya, A., & Santiago, J. G. (2024). Design and Evaluation of a Robust CRISPR Kinetic Assay for Hot-Spot Genotyping. Analytical Chemistry, 96(19), 7444-7451.

Ramachandran, A., & Santiago, J. G. (2021). CRISPR enzyme kinetics for molecular diagnostics. Analytical chemistry, 93(20), 7456-7464.

Huyke, D. A., Ramachandran, A., Bashkirov, V. I., Kotseroglou, E. K., Kotseroglou, T., & Santiago, J. G. (2022). Enzyme kinetics and detector sensitivity determine limits of detection of amplification-free CRISPR-Cas12 and CRISPR-Cas13 diagnostics. Analytical Chemistry, 94(27), 9826-9834.